Effects of plasmin on human factor VIII (AHF).

Highly purified, fibrinogen-free human gel electrophoresis. The split products factor VIII was incubated with plasmin, were not anticoagulant in the factor and the liberated split products of the VIII activity assay. In fact, the breakfactor VIII were analyzed by gel filtradown products in the hydrolysate intion, acrylamide gel electrophoresis, creased the factor VIII activity of bioassay, and for immunologic reactinormal plasma mixed with it. Therevity. At least three fragments retaining fore, it is not likely that the factor VIII different antigenic determinants are split products formed in fibrinolytic released from the factor VIII after states contribute actively to the hemorprolonged digestion and at least three rhagic diathesis. new fragments are seen in acrylamide